| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.116936 |
| Chromosome: | chromosome 3 |
| Location: | 3018403 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g163900 | PWR3 | (1 of 14) PF04720 - PDDEXK-like family of unknown function (PDDEXK_6); PWR motif protein | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AATCGGAAGCCGTGCCGCACGTTCCACAAA |
| Internal bar code: | CCAAACCGGCGTGACAGGCTCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 548 |
| LEAP-Seq percent confirming: | 99.483 |
| LEAP-Seq n confirming: | 2309 |
| LEAP-Seq n nonconfirming: | 12 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTGTGTCCATGCCTGTTTGT |
| Suggested primer 2: | ATCAACAACAAGGAGGTGGC |