| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.116956 |
| Chromosome: | chromosome 13 |
| Location: | 430373 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g564766 | (1 of 20) 3.1.4.17 - 3',5'-cyclic-nucleotide phosphodiesterase / Cyclic AMP phosphodiesterase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCGGGAGGTAGCGGGGCCCTCGGCCCCCT |
| Internal bar code: | GAGGGACAGGTTTCCCCAGTAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 830 |
| LEAP-Seq percent confirming: | 97.9263 |
| LEAP-Seq n confirming: | 850 |
| LEAP-Seq n nonconfirming: | 18 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTCGCTATAGCCACTTTGCC |
| Suggested primer 2: | ACTCAAGGTTCGCTGCTGTT |