| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.116964 |
| Chromosome: | chromosome 13 |
| Location: | 3224678 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g585700 | (1 of 42) IPR000008 - C2 domain | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGCGTGTGTGGGGTGGGATACGGCGGGGGT |
| Internal bar code: | GCGAAGGGACGAGAGCGAGGCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 130 |
| LEAP-Seq percent confirming: | 97.1129 |
| LEAP-Seq n confirming: | 370 |
| LEAP-Seq n nonconfirming: | 11 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAAGCGGGGGAAAAGAGTTA |
| Suggested primer 2: | AGCAAGAGTTGCTTTTCCCA |