Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | - |
Strain: | LMJ.RY0402.116990 |
Chromosome: | chromosome 1 |
Location: | 1544315 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre01.g008000 | (1 of 1) K18666 - activating signal cointegrator complex subunit 1 (ASCC1) | intron |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGCATTGCGCATGTACGTCCTCGGGCTGG |
Internal bar code: | CGATGCAGGTCGCTCGCGAGAA |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 738 |
LEAP-Seq percent confirming: | 99.8348 |
LEAP-Seq n confirming: | 4231 |
LEAP-Seq n nonconfirming: | 7 |
LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | ATAATGGTAAGCACAGGGCG |
Suggested primer 2: | CTGTTACGCACACATCCCAC |