Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | - |
Strain: | LMJ.RY0402.116995 |
Chromosome: | chromosome_5 |
Location: | 1323959 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre05.g244800 | NUOAF3 | antisense | 3'UTR |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | GATTTGATGTCGACTGGAGGCACCCTCACC |
Internal bar code: | AAATGGTCTGGTTTTATCAAGT |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 873 |
LEAP-Seq percent confirming: | 99.735 |
LEAP-Seq n confirming: | 1882 |
LEAP-Seq n nonconfirming: | 5 |
LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GAAGAAGAACCCTGTGAGCG |
Suggested primer 2: | TTACATCTTGCAACCCCTCC |