| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.117027 |
| Chromosome: | chromosome 7 |
| Location: | 320343 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g314400 | (1 of 1) PF00580//PF13538 - UvrD/REP helicase N-terminal domain (UvrD-helicase) // UvrD-like helicase C-terminal domain (UvrD_C_2) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCAGTCCTGACTCCCTCACCATCAGGATG |
| Internal bar code: | TCAAAATGCCTCTCGCTACCGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 651 |
| LEAP-Seq percent confirming: | 99.8678 |
| LEAP-Seq n confirming: | 4532 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 22 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCCAGTGTGTGGTGGTCAGT |
| Suggested primer 2: | TTCTCTCTCCTCCTCCTCCC |