| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.117109 |
| Chromosome: | chromosome 12 |
| Location: | 7225532 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g558350 | (1 of 1) PF02609 - Exonuclease VII small subunit (Exonuc_VII_S) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGGATGGCACACCACCGCGTCCCACGTGC |
| Internal bar code: | CCGACTCAAGCGCAACGCAGTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 592 |
| LEAP-Seq percent confirming: | 94.3627 |
| LEAP-Seq n confirming: | 385 |
| LEAP-Seq n nonconfirming: | 23 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAAACTGGTGTCCCCTGAGT |
| Suggested primer 2: | GAGCTCATGATCTTTCCCCA |