| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.117159 |
| Chromosome: | chromosome 12 |
| Location: | 9298858 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g541100 | OTU7 | (1 of 1) PTHR12419//PTHR12419:SF19 - OTU DOMAIN CONTAINING PROTEIN // SUBFAMILY NOT NAMED; OTU-like cysteine protease | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGCACCTTCTGTTCTAGTATTGGTACAAAC |
| Internal bar code: | GTCGTGTCTTAGCGATAGTGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 483 |
| LEAP-Seq percent confirming: | 99.8219 |
| LEAP-Seq n confirming: | 3362 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 23 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCCTCGCTCCTTTAGTCCTT |
| Suggested primer 2: | ACATGTGCTGCATGTGACCT |