| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.117212 |
| Chromosome: | chromosome 7 |
| Location: | 4871565 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g346100 | (1 of 1) 3.1.21.7 - Deoxyribonuclease V / Escherichia coli endodeoxyribonuclease V | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCTACGCACACAATACGGCCACTCCCTCTC |
| Internal bar code: | CCCTCGGGCCTGTACTCCCGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 823 |
| LEAP-Seq percent confirming: | 99.1987 |
| LEAP-Seq n confirming: | 4209 |
| LEAP-Seq n nonconfirming: | 34 |
| LEAP-Seq n unique pos: | 14 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AACAGCTACGGACCATCCAC |
| Suggested primer 2: | AAGCTTACGACGAATCCCCT |