| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.117237 |
| Chromosome: | chromosome 10 |
| Location: | 4506814 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g452000 | (1 of 1) PF04752 - ChaC-like protein (ChaC) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGTGCGACCCTTTCCAGTGCCACGACCTG |
| Internal bar code: | TTACGAGCTCGGCCGTCTAGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 819 |
| LEAP-Seq percent confirming: | 99.8436 |
| LEAP-Seq n confirming: | 3830 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACCGAGACTGGGTGATAACG |
| Suggested primer 2: | CACCTGAACAGGTACTCGCA |