| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.117237 |
| Chromosome: | chromosome 10 |
| Location: | 4506822 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g452000 | (1 of 1) PF04752 - ChaC-like protein (ChaC) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGAAGGGGCTTGCACAGGGCATGTATGGA |
| Internal bar code: | ACCGGAAATTTCATACTTGTCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 850 |
| LEAP-Seq percent confirming: | 99.754 |
| LEAP-Seq n confirming: | 2433 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 25 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACCGAGACTGGGTGATAACG |
| Suggested primer 2: | CACCTGAACAGGTACTCGCA |