Insertion junction: LMJ.RY0402.117288_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.117288
Chromosome:chromosome 17
Location:6125387
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre17.g741500 KIL7 Putative kinesin-like motor protein; (1 of 1) 3.4.21.72//3.6.4.4 - IgA-specific serine endopeptidase / Immunoglobulin A1 protease // Plus-end-directed kinesin ATPase / Kinesin CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TCCTCGAGCATGTTCTTCTGCTGCTTGAGC
Internal bar code:GGAGCAGGTAGATGGTTGCTTG

Confirmation - LEAP-Seq

LEAP-Seq distance:965
LEAP-Seq percent confirming:99.7441
LEAP-Seq n confirming:2339
LEAP-Seq n nonconfirming:6
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:GTGTGTGCACAAGCAGGTCT
Suggested primer 2:GTCGCATGGTAAGCTGGTTT