| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.117294 |
| Chromosome: | chromosome 6 |
| Location: | 7284698 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g298550 | FMO7 | Flavin-containing monooxygenase; (1 of 3) K00485 - dimethylaniline monooxygenase (N-oxide forming) (FMO) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGGCGGTGCCGTGGAACACTCCACAGTCG |
| Internal bar code: | GTCAGAAGCGTCTAGGGGGTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1 |
| LEAP-Seq percent confirming: | 99.6418 |
| LEAP-Seq n confirming: | 3338 |
| LEAP-Seq n nonconfirming: | 12 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGAGTATGCGAGGCTCTGTC |
| Suggested primer 2: | CTATCAGGGACTCAGCCAGC |