Insertion junction: LMJ.RY0402.117306_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.117306
Chromosome:chromosome 9
Location:2661072
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre09.g389950 OAT1 O-acetyltransferase-related protein; (1 of 1) 2.3.1.45 - N-acetylneuraminate 7-O(or 9-O)-acetyltransferase 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TAAACGCAAAGCGCTACCCAGCCACGTCGG
Internal bar code:CGACGACGGTTGTGGACCCGAG

Confirmation - LEAP-Seq

LEAP-Seq distance:304
LEAP-Seq percent confirming:99.7915
LEAP-Seq n confirming:2393
LEAP-Seq n nonconfirming:5
LEAP-Seq n unique pos:8

Suggested primers for confirmation by PCR

Suggested primer 1:CTGACCAACACGCTGAAGAA
Suggested primer 2:CTAAGGAGGACGCACTGGAG