Insertion junction: LMJ.RY0402.117308_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.117308
Chromosome:chromosome 3
Location:8135941
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre03.g200700 FAP166 (1 of 79) IPR011992 - EF-hand domain pair; Flagellar Associated Protein 166 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTCCAGTCGCGGCTACTCACGTCCAGGGCC
Internal bar code:ACGGGGCCTAGATATGAAACTC

Confirmation - LEAP-Seq

LEAP-Seq distance:116
LEAP-Seq percent confirming:91.6866
LEAP-Seq n confirming:4974
LEAP-Seq n nonconfirming:451
LEAP-Seq n unique pos:20

Suggested primers for confirmation by PCR

Suggested primer 1:CAGCTGCAGGTAGTTGATGC
Suggested primer 2:TGGTTGACACGACCATCACT