Insertion junction: LMJ.RY0402.117354_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.117354
Chromosome:chromosome 7
Location:6336797
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre07.g357250 protein of unknown function; (1 of 1) PTHR24188//PTHR24188:SF41 - ANKYRIN REPEAT PROTEIN // SUBFAMILY NOT NAMED CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTTGGCGCTCACGCAGTCCAGCCCGTCCGC
Internal bar code:TAGTGCGGCAACGTGGTTACC

Confirmation - LEAP-Seq

LEAP-Seq distance:453
LEAP-Seq percent confirming:84.4492
LEAP-Seq n confirming:1564
LEAP-Seq n nonconfirming:288
LEAP-Seq n unique pos:7

Suggested primers for confirmation by PCR

Suggested primer 1:AGAAACACCTGTCCGTGCTC
Suggested primer 2:GCTGGTTGTGATGTTGAGGA