| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.117358 |
| Chromosome: | chromosome 12 |
| Location: | 291331 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g485550 | (1 of 1) 4.1.3.4 - Hydroxymethylglutaryl-CoA lyase / HMG-CoA lyase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACAACGATTTGCAAGCGCTCTGTGCGGCAG |
| Internal bar code: | TTGACTTGCAGACGATTTTCAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 949 |
| LEAP-Seq percent confirming: | 99.4709 |
| LEAP-Seq n confirming: | 1504 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCACTGCTTTGCTTTCTTCC |
| Suggested primer 2: | GCTCGTAAAACCTAGCGTGC |