| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.117365 |
| Chromosome: | chromosome 1 |
| Location: | 5750828 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g041050 | AOC6 | Cationic amino acid transporter; (1 of 2) K03294 - basic amino acid/polyamine antiporter, APA family (TC.APA) | intron|outside_mRNA |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAAAAATTGTGCCAACGCTAACTACCGTAT |
| Internal bar code: | ATGGAGGGGTCTGAGCGGGACG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 621 |
| LEAP-Seq percent confirming: | 99.5346 |
| LEAP-Seq n confirming: | 1497 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTGGTAGAGGTGGGATGAGG |
| Suggested primer 2: | TTAAGGCACGCTACATGCTG |