| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.117401 |
| Chromosome: | chromosome 3 |
| Location: | 9032787 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g210177 | (1 of 3) PF13405//PF13499 - EF-hand domain (EF-hand_6) // EF-hand domain pair (EF-hand_7) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCCTCATCGGATAGGTCCAGGCCGAGGTT |
| Internal bar code: | TCACAACGTCGTCACTCGACC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 462 |
| LEAP-Seq percent confirming: | 92.2658 |
| LEAP-Seq n confirming: | 1694 |
| LEAP-Seq n nonconfirming: | 142 |
| LEAP-Seq n unique pos: | 17 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTCATGGAGATGTGGTTGGA |
| Suggested primer 2: | TAAGCTGGAAGAAGGGGGAT |