Insertion junction: LMJ.RY0402.117402_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.117402
Chromosome:chromosome 3
Location:1483236
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre03.g151600 (1 of 29) IPR029033 - Histidine phosphatase superfamily intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACTTTACGTGTGACGCGGGCTGACCACCGG
Internal bar code:GTGGAAGACTGGGTCATGCGCG

Confirmation - LEAP-Seq

LEAP-Seq distance:946
LEAP-Seq percent confirming:98.3894
LEAP-Seq n confirming:4154
LEAP-Seq n nonconfirming:68
LEAP-Seq n unique pos:20

Suggested primers for confirmation by PCR

Suggested primer 1:ACAGGCTGGATTGTTTACGG
Suggested primer 2:AGCGCATCTACACAGTGGTG