| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.117408 |
| Chromosome: | chromosome 9 |
| Location: | 7262737 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g412600 | CYG52 | (1 of 1) PF00072//PF00211 - Response regulator receiver domain (Response_reg) // Adenylate and Guanylate cyclase catalytic domain (Guanylate_cyc); Adenylate/guanylate cyclase | CDS/intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTTGATGCTCCGCTTCAGCGCCAGCTGTGT |
| Internal bar code: | GTCGTAGACCGAGCAAGGGGCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 369 |
| LEAP-Seq percent confirming: | 99.8279 |
| LEAP-Seq n confirming: | 12764 |
| LEAP-Seq n nonconfirming: | 22 |
| LEAP-Seq n unique pos: | 29 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAATGCGTGTGGAATGTGTC |
| Suggested primer 2: | GAGTACGAAGTGCTGGAGGC |