Insertion junction: LMJ.RY0402.117419_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.117419
Chromosome:chromosome 2
Location:2248087
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre02.g090050 FAP285,FAP170 Flagellar Associated Protein 170 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGTGGTTGAGGCTCAGTCGGCGGCCAGCGC
Internal bar code:ACCAGGCCGGAGCTTTCATGTG

Confirmation - LEAP-Seq

LEAP-Seq distance:326
LEAP-Seq percent confirming:98.9011
LEAP-Seq n confirming:450
LEAP-Seq n nonconfirming:5
LEAP-Seq n unique pos:4

Suggested primers for confirmation by PCR

Suggested primer 1:CTCGGAGTTTGGAAATGGAA
Suggested primer 2:ATGGGGTTGGGTATGTCGTA