| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.117420 |
| Chromosome: | chromosome_8 |
| Location: | 3500196 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre08.g375050 | FAP86 | Flagellar Associated Protein | sense | CDS |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | AGGGTGAGCTGTCAGCGCGCACTCTGCTGA |
| Internal bar code: | GAAGACGCTATACTCCAGGTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 430 |
| LEAP-Seq percent confirming: | 96.6667 |
| LEAP-Seq n confirming: | 29 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTTTACGCCACCTTCGTGAT |
| Suggested primer 2: | CCGAAACGCCGTAGAAATTA |