| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.117420 |
| Chromosome: | chromosome_8 |
| Location: | 3500259 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre08.g375050 | FAP86 | Flagellar Associated Protein | antisense | 5'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | ATGGGGGCTTCTTGTTTTACTTTTAGTGTC |
| Internal bar code: | CGGGTCGTCCGCCCTCTTACCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 787 |
| LEAP-Seq percent confirming: | 98.9303 |
| LEAP-Seq n confirming: | 2682 |
| LEAP-Seq n nonconfirming: | 29 |
| LEAP-Seq n unique pos: | 16 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCGAAACGCCGTAGAAATTA |
| Suggested primer 2: | GTTTACGCCACCTTCGTGAT |