| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.117438 |
| Chromosome: | chromosome 4 |
| Location: | 2794886 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g223900 | (1 of 2) K09313 - homeobox protein cut-like (CUTL) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCCAGCCGCCCGACACACGTTGACCCAGC |
| Internal bar code: | CTCTGCGTTCATTAAAACAGTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 88 |
| LEAP-Seq percent confirming: | 81.8182 |
| LEAP-Seq n confirming: | 9 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGTGTTGCTCTCCTGTGTGT |
| Suggested primer 2: | GCCCACCACTCACTCTTCTC |