| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.117463 |
| Chromosome: | chromosome 13 |
| Location: | 4941954 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g606200 | TMC5 | (1 of 1) 2.1.1.176//2.1.1.178 - 16S rRNA (cytosine(967)-C(5))-methyltransferase // 16S rRNA (cytosine(1407)-C(5))-methyltransferase; putative tRNA (cytosine-5)-methyltransferase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGCTCACGGCGCTCAGACTGCCATCCTGC |
| Internal bar code: | CGCTTTCTCAGGGCGAGTTGTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 420 |
| LEAP-Seq percent confirming: | 99.8385 |
| LEAP-Seq n confirming: | 1855 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCCTCAGTATCCTGTTCCCA |
| Suggested primer 2: | CCTACTGACGCAGTTCACGA |