| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.117465 |
| Chromosome: | chromosome 10 |
| Location: | 5978163 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g462450 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CACGAAGGCAAAGGCGCACTCCGATGGCTT |
| Internal bar code: | GAGTCCTAGCCCATTGGCAACG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 720 |
| LEAP-Seq percent confirming: | 99.7027 |
| LEAP-Seq n confirming: | 1006 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCGTCAATAGCGGTAATGGT |
| Suggested primer 2: | TGAACTGTTATGGCTGCTGG |