Insertion junction: LMJ.RY0402.117469_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.117469
Chromosome:chromosome 9
Location:2069515
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre09.g394050 CPLD19 Zinc finger family protein; (1 of 1) PTHR22883:SF91 - PROTEIN S-ACYLTRANSFERASE 12-RELATED CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GAGGAGATATTCGGGCGCAAGTGCGTAGGG
Internal bar code:GGCACCGTATGATAGCTGAGCA

Confirmation - LEAP-Seq

LEAP-Seq distance:42
LEAP-Seq percent confirming:97.5
LEAP-Seq n confirming:39
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:TGTCTGTGTCTCCAACGCTC
Suggested primer 2:GAAGGAAGGAAGGGGAACAG