| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.117479 |
| Chromosome: | chromosome 13 |
| Location: | 1722901 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g574400 | CYG55 | (1 of 11) 4.6.1.1//4.6.1.2 - Adenylate cyclase / ATP pyrophosphate-lyase // Guanylate cyclase / Guanylyl cyclase; Adenylate/guanylate cyclase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGGTTCCTGCTGATCTAGTAGCGCAGGAG |
| Internal bar code: | CGACAATTTTCCATGCCGGGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 293 |
| LEAP-Seq percent confirming: | 79.4254 |
| LEAP-Seq n confirming: | 3262 |
| LEAP-Seq n nonconfirming: | 845 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTCCACAGCAATTAGCAGCA |
| Suggested primer 2: | TCGCAGCCTCAGTCTCAGTA |