| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.117516 |
| Chromosome: | chromosome 3 |
| Location: | 8259233 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g199400 | ORC2 | Origin recognition complex subunit 2; (1 of 1) K02604 - origin recognition complex subunit 2 (ORC2) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCAACACACCCATCCAGCCACAACCACCGC |
| Internal bar code: | GCACCTGGCTAGCAGACGCTAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 195 |
| LEAP-Seq percent confirming: | 98.6766 |
| LEAP-Seq n confirming: | 1715 |
| LEAP-Seq n nonconfirming: | 23 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCCCATCCTCTCCTCATGTC |
| Suggested primer 2: | GAATCCAGCCGCAATACTGT |