Insertion junction: LMJ.RY0402.117519_4

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.117519
Chromosome:chromosome 12
Location:7881830
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre12.g552750 (1 of 2) PF04520 - Senescence regulator (Senescence_reg) 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCTTCAACGCCTTAGTTTCTGACGGTTCCA
Internal bar code:CGTAGCTTGAGTGCGCGTCGCC

Confirmation - LEAP-Seq

LEAP-Seq distance:640
LEAP-Seq percent confirming:97.6331
LEAP-Seq n confirming:165
LEAP-Seq n nonconfirming:4
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:AGGTGCATATAGGGCCTGTG
Suggested primer 2:ACTCATTCATACCCCACCCA