| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.117524 |
| Chromosome: | chromosome 10 |
| Location: | 185243 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g419000 | (1 of 1) PTHR13105//PTHR13105:SF7 - MYELOID LEUKEMIA FACTOR // MYELOID LEUKEMIA FACTOR | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGATGCAGCGGGCGTACCATCGTCGGGTT |
| Internal bar code: | TCCCCCCGTGCAGCCTCACACC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 410 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 1 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAGACAACGGGAGAGACAGC |
| Suggested primer 2: | TGAACATGAAGCTACTGCCG |