| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.117533 |
| Chromosome: | chromosome_17 |
| Location: | 512236 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre17.g699500 | TTL8,TTLL9,FAP267 | Tubulin tyrosine ligase | antisense | intron |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | GTAGGTGTTGGTGATGTCCTCCTTGCGAGT |
| Internal bar code: | GAAGGTGAGCGAAAATGGACC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 58 |
| LEAP-Seq percent confirming: | 99.8221 |
| LEAP-Seq n confirming: | 561 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGGAGGGTTGGAGAGGTGTT |
| Suggested primer 2: | AGTCCCCTCTGTGTGTTTGG |