| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.117535 |
| Chromosome: | chromosome 13 |
| Location: | 2442258 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g579800 | (1 of 1) 2.7.4.26 - Isopentenyl phosphate kinase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGTTCCGCGCCTGCGGGCCGAGCTTGGCAA |
| Internal bar code: | TTTGCAGGACATGTACGCGGTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 755 |
| LEAP-Seq percent confirming: | 99.911 |
| LEAP-Seq n confirming: | 1123 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 15 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATGTGTTGTCCTAGGGGCAG |
| Suggested primer 2: | CCAGCATCCTTTTCAAGCTC |