Insertion junction: LMJ.RY0402.117569_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.117569
Chromosome:chromosome 16
Location:3894168
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre16.g690350 SNR6 SNARE-associated Golgi protein; (1 of 1) PTHR12677:SF24 - TRANSMEMBRANE PROTEIN 64 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CGCGACCGCCTTCTCTACCCTCGGCAGCCG
Internal bar code:TCGTGTATTGGAGCTGCGTCAA

Confirmation - LEAP-Seq

LEAP-Seq distance:448
LEAP-Seq percent confirming:99.6359
LEAP-Seq n confirming:5200
LEAP-Seq n nonconfirming:19
LEAP-Seq n unique pos:13

Suggested primers for confirmation by PCR

Suggested primer 1:CCGTGCCAGTTGATGTATTG
Suggested primer 2:CAACGGAGCTAGAGAGGGTG