| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.117575 |
| Chromosome: | chromosome 13 |
| Location: | 3766417 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g589600 | (1 of 100) IPR001810 - F-box domain | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTCGGGGGTGTTTGTAGTGCGGCCAATGTT |
| Internal bar code: | CGTCCCGCAGGCCGCGCGCTTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 853 |
| LEAP-Seq percent confirming: | 97.4838 |
| LEAP-Seq n confirming: | 1201 |
| LEAP-Seq n nonconfirming: | 31 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGGTCGTCAGCTCTCTCATC |
| Suggested primer 2: | AGCGACCCACACCAGAATAC |