Insertion junction: LMJ.RY0402.117577_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.117577
Chromosome:chromosome 6
Location:1966141
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre06.g263850 PPT2,TPT2,TPT20 Triose phosphate/phosphate translocator; (1 of 2) PTHR11132:SF70 - PHOSPHOENOLPYRUVATE/PHOSPHATE TRANSLOCATOR 1, CHLOROPLASTIC 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTGTCCCGCATCCGGCATCCCCGTATCGCA
Internal bar code:TCGCTGCGGCGATCCAAAGCTC

Confirmation - LEAP-Seq

LEAP-Seq distance:680
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:14
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:TTAGCATTTCGACTTCGGCT
Suggested primer 2:TGTCTCAACCGCATCTTCTG