| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.117588 |
| Chromosome: | chromosome 11 |
| Location: | 2180314 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g469800 | (1 of 11) PF07173 - Protein of unknown function (DUF1399) (DUF1399) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCTGAGCGGCTGAGCCACATCAGCTCGTGA |
| Internal bar code: | GTGATCGGCTGCACGCGACGTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 651 |
| LEAP-Seq percent confirming: | 99.8605 |
| LEAP-Seq n confirming: | 4294 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 22 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGCCCCTTCTCTAAATCCC |
| Suggested primer 2: | CATGTCATTGGAGCCAGTTG |