| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.117659 |
| Chromosome: | chromosome 13 |
| Location: | 4728564 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g604750 | CGL94 | zinc finger family protein; (1 of 37) PF13639 - Ring finger domain (zf-RING_2) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCTGGGTGTGTAGTGGCACATGGCGAGCCG |
| Internal bar code: | GGGCCGGCTTTTTTGGGGCCAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 836 |
| LEAP-Seq percent confirming: | 98.7952 |
| LEAP-Seq n confirming: | 902 |
| LEAP-Seq n nonconfirming: | 11 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACTCTCTTTGGCCTATCGCA |
| Suggested primer 2: | CTGAGTACAGCTGTGCGAGC |