| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.117659 |
| Chromosome: | chromosome 13 |
| Location: | 4728778 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g604750 | CGL94 | zinc finger family protein; (1 of 37) PF13639 - Ring finger domain (zf-RING_2) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCAGGTGTGGCGCAATCTTGCTGCTGTTGC |
| Internal bar code: | TACCCCGTCCCGCGGCATCGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 696 |
| LEAP-Seq percent confirming: | 99.8439 |
| LEAP-Seq n confirming: | 4477 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 19 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCAAGCTTCTTCCCTGCAAT |
| Suggested primer 2: | TTCTCCTGTTGCGACACTTG |