| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.117714 |
| Chromosome: | chromosome 6 |
| Location: | 1542799 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g260000 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACGCACAAGTCCTCGGCATGGCTAAATAAA |
| Internal bar code: | ACGAAAGAAGGTGCGAAACGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 702 |
| LEAP-Seq percent confirming: | 99.9204 |
| LEAP-Seq n confirming: | 1255 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTTCATCTTCTCCAGCAGGC |
| Suggested primer 2: | TGGCGTGGCTTCTATAGCTT |