| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.117716 |
| Chromosome: | chromosome 17 |
| Location: | 7068737 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g746997 | ADHE,ADHE1,ADH1 | Dual function alcohol/acetaldehyde dehydrogenase; (1 of 2) 1.2.1.10 - Acetaldehyde dehydrogenase (acetylating) / Aldehyde dehydrogenase (acylating) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATTCGGGCTGAGCGATGCCCCTGTAGCGGC |
| Internal bar code: | TCGGCATGTCGTCCCGATATAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 993 |
| LEAP-Seq percent confirming: | 99.53 |
| LEAP-Seq n confirming: | 1694 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACCCGCGCTGATGTATAAAG |
| Suggested primer 2: | CGTAGAGGCGCGGTACTTAG |