| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.117736 |
| Chromosome: | chromosome 7 |
| Location: | 3484387 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g335650 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGGAGGGGGACGGGGCAACGTGTTACAAAC |
| Internal bar code: | GCTCCCTGGCCGGCGGGTGCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 892 |
| LEAP-Seq percent confirming: | 99.8431 |
| LEAP-Seq n confirming: | 3818 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 21 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGTATGCCACGTCAGAGCTA |
| Suggested primer 2: | CTGCTCTCGACACTGCTTTG |