| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.117757 |
| Chromosome: | chromosome 16 |
| Location: | 1340462 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g651801 | (1 of 7) IPR000104//IPR020683 - Antifreeze protein, type I // Ankyrin repeat-containing domain | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCTCTTCGTGGACCGCAAATATGCACACAC |
| Internal bar code: | TTCTCCTAGGCAGGGGTTCAAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 509 |
| LEAP-Seq percent confirming: | 99.7627 |
| LEAP-Seq n confirming: | 841 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCTGCGATAGTGCTTGTTGA |
| Suggested primer 2: | GACAGGAGTTCCTCAGCGAC |