Insertion junction: LMJ.RY0402.117829_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.117829
Chromosome:chromosome 2
Location:5662989
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre02.g109683 (1 of 3) IPR002912//IPR011598 - ACT domain // Myc-type, basic helix-loop-helix (bHLH) domain intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TCGTCATACATATCAGATCAACGTATCCCG
Internal bar code:GGACCGACGAAACTGCGGTAAA

Confirmation - LEAP-Seq

LEAP-Seq distance:670
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:167
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:5

Suggested primers for confirmation by PCR

Suggested primer 1:GTGGTTCTGGCGGACACTAT
Suggested primer 2:CCATGGGATGGTGTGTATCA