| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.117845 |
| Chromosome: | chromosome 4 |
| Location: | 1286630 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g214800 | VMPL1,VMP6 | (1 of 10) PF00957 - Synaptobrevin (Synaptobrevin); Endosomal R-SNARE protein, VAMP-like family (R.III) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGCGCAACAAAGGGAGAGCGTGTGGGCGT |
| Internal bar code: | GCTTCTCCGTTCTCACCCCACG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 841 |
| LEAP-Seq percent confirming: | 99.2509 |
| LEAP-Seq n confirming: | 795 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGATGCGTGGGAATAAGACA |
| Suggested primer 2: | TATGGACGTGTGATGGCAGT |