| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.117861 |
| Chromosome: | chromosome 3 |
| Location: | 4414215 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g175600 | (1 of 1) PTHR26392//PTHR26392:SF78 - MITOGEN-ACTIVATED PROTEIN KINASE KINASE KINASE 7-RELATED // SUBFAMILY NOT NAMED | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTCCCTGCAGTGGGCCGCAGTAGCTGCCAA |
| Internal bar code: | CGTGCCTCCCTACTCGCGGCTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 338 |
| LEAP-Seq percent confirming: | 99.1031 |
| LEAP-Seq n confirming: | 221 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAAGCTACGGAACCTCAAGC |
| Suggested primer 2: | ATCGTGGAATGGAACTCAGC |