| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.117887 |
| Chromosome: | chromosome 17 |
| Location: | 953960 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g703000 | (1 of 5) 3.2.1.3 - Glucan 1,4-alpha-glucosidase / Lysosomal alpha-glucosidase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACACGGTGGCTACTTCCTGGTCGCAGTATC |
| Internal bar code: | GGTCCGGGCAGACATTGAGCAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 687 |
| LEAP-Seq percent confirming: | 99.0517 |
| LEAP-Seq n confirming: | 2089 |
| LEAP-Seq n nonconfirming: | 20 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACAAGCCTCTGCTCTACCC |
| Suggested primer 2: | GTCGCTGTGTGAATGTGCTT |