| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.117918 |
| Chromosome: | chromosome 5 |
| Location: | 3262627 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g240225 | (1 of 1) IPR008754//IPR024079 - Peptidase M43, pregnancy-associated plasma-A // Metallopeptidase, catalytic domain | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCACCCTCGGCGCCTGCGCAACTCTTCAAG |
| Internal bar code: | AAAACCCCGCGACCCCATTTAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 524 |
| LEAP-Seq percent confirming: | 99.8917 |
| LEAP-Seq n confirming: | 922 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 14 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCTATGCCATCTGCGTCAAG |
| Suggested primer 2: | AGCCGTACCATCAGCCATAC |