Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | - |
Strain: | LMJ.RY0402.117918 |
Chromosome: | chromosome_5 |
Location: | 3262627 |
Confidence (%): | 58 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre05.g240225 | antisense | intron |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | GCACCCTCGGCGCCTGCGCAACTCTTCAAG |
Internal bar code: | AAAACCCCGCGACCCCATTTAC |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 524 |
LEAP-Seq percent confirming: | 99.8917 |
LEAP-Seq n confirming: | 922 |
LEAP-Seq n nonconfirming: | 1 |
LEAP-Seq n unique pos: | 14 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | TCTATGCCATCTGCGTCAAG |
Suggested primer 2: | AGCCGTACCATCAGCCATAC |