| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.117929 |
| Chromosome: | chromosome 9 |
| Location: | 6274103 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g406448 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTGGCTATCACATGCTTCGTGCCGCATCCA |
| Internal bar code: | GGCCGTCGTAGCGTTGTAAAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 12 |
| LEAP-Seq percent confirming: | 86.3636 |
| LEAP-Seq n confirming: | 19 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGAGCACGGTCATGTTTAGA |
| Suggested primer 2: | CGTAACACCCCTCATCTCGT |